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FHL2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84978

Novus Biologicals, part of Bio-Techne
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NBP1-84978
NBP1-84978-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSS

Reactivity Notes

Reactivity reported in scientific literature (PMID: 20029370)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FHL2 Antibody

Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978]

Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978]

Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Analysis in human heart muscle and liver tissues. Corresponding FHL2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978]

Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978]

Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Staining of human cerebellum, heart muscle, liver and skeletal muscle using Anti-FHL2 antibody NBP1-84978 (A) shows similar protein distribution across tissues to independent antibody NBP1-84979 (B).
Western Blot: FHL2 Antibody [NBP1-84978]

Western Blot: FHL2 Antibody [NBP1-84978]

Western Blot: FHL2 Antibody [NBP1-84978] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for FHL2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FHL2

Long Name

Four and a Half LIM Domains Protein 2

Alternate Names

AAG11, DRAL, SLIM3

Gene Symbol

FHL2

Additional FHL2 Products

Product Documents for FHL2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FHL2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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