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FHL3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04622

Novus Biologicals, part of Bio-Techne
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NBP3-04622-100ul
NBP3-04622-20ul

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human FHL3 (NP_004459.2). MSESFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FHL3 Antibody - BSA Free

Western Blot: FHL3 AntibodyBSA Free [NBP3-04622]

Western Blot: FHL3 AntibodyBSA Free [NBP3-04622]

Western Blot: FHL3 Antibody [NBP3-04622] - Analysis of extracts of various cell lines, using FHL3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Immunocytochemistry/ Immunofluorescence: FHL3 Antibody - BSA Free [NBP3-04622]

Immunocytochemistry/ Immunofluorescence: FHL3 Antibody - BSA Free [NBP3-04622]

Immunocytochemistry/Immunofluorescence: FHL3 Antibody [NBP3-04622] - Immunofluorescence analysis of HeLa cells using FHL3 Rabbit pAb (NBP3-04622) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for FHL3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FHL3

LIM proteins are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Alternate Names

FHL-3, four and a half LIM domains 3, four and a half LIM domains protein 3, LIM-only protein FHL3, MGC19547, MGC8696, Skeletal muscle LIM-protein 2, SLIM-2, SLIM2MGC23614

Gene Symbol

FHL3

Additional FHL3 Products

Product Documents for FHL3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FHL3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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