Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14728
Recombinant Monoclonal
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR150J
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
residues 26-760 (ECD)
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
![Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (BLR150J) [NBP3-14728]](https://resources.bio-techne.com/images/products/Fibroblast-Activation-Protein-alpha-FAP-Antibody-Immunoprecipitation-NBP3-14728-img0001.jpg "Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (BLR150J) [NBP3-14728]")
Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (BLR150J) [NBP3-14728]
Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Whole cell lysate (500 ug per IP reaction; 5% of IPloaded) from IMR-90 cells. Antibody: Rabbit anti-FAPrecombinant monoclonal antibody used for IP at 20 ul/mg lysate. For blottingimmunoprecipitated FAP, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.
Western Blot-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -
Western Blot-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -Whole cell lysate (50 ug) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti FAP recombinant monoclonal antibody [BLR150J] used at 1:1000. Secondary: HRP conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Immunohistochemistry-Paraffin-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -
Immunohistochemistry-Paraffin-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Rabbit anti-FibroblastActivation Protein alpha/FAP recombinant monoclonalantibody [BLR150J]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate:DAB.Applications for Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 to 1:500
Immunohistochemistry
1:100 to 1:500
Immunohistochemistry-Paraffin
1:100 to 1:500
Immunoprecipitation
20 µl/1 mg lysate
Western Blot
1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: Fibroblast Activation Protein alpha/FAP
Alternate Names
FAP, Seprase
Gene Symbol
FAP
Additional Fibroblast Activation Protein alpha/FAP Products
- All Products for Fibroblast Activation Protein alpha/FAP
- Fibroblast Activation Protein alpha/FAP cDNA Clones
- Fibroblast Activation Protein alpha/FAP ELISA Kits
- Fibroblast Activation Protein alpha/FAP Lysates
- Fibroblast Activation Protein alpha/FAP Primary Antibodies
- Fibroblast Activation Protein alpha/FAP Proteins and Enzymes
- Fibroblast Activation Protein alpha/FAP Simple Plex
Product Documents for Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
Product Specific Notices for Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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