Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # ER-TR7

Format

BSA Free

Concentration

1.0 mg/ml

View all Fibroblast Antibodies »

Product Specifications

Immunogen

Isolated C3H thymic stromal cells

Specificity

NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. Clone ER-TR7 has been used to stain the microanatomy of various organs and also stains subendothelial deposits in atherosclerotic plaques.

Marker

Fibroblast Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Fibroblast Antibody (ER-TR7) - BSA Free

Immunohistochemistry: Fibroblast Antibody (ER-TR7) - BSA Free [NB100-64932]

Immunohistochemistry: Fibroblast Antibody (ER-TR7) - BSA Free [NB100-64932]

Immunohistochemistry: Fibroblast Antibody (ER-TR7) [NB100-64932] - (Ai-Aii) Representative ER-TR7 stained sections from xenograft tumors harvested at day 7. (Ai) Untreated, (Aii) Pt. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/full/10.1002/jbm.b.34254), licensed under a CC-BY license.
Immunohistochemistry-Frozen: Fibroblast Antibody (ER-TR7) - BSA Free [NB100-64932]

Immunohistochemistry-Frozen: Fibroblast Antibody (ER-TR7) - BSA Free [NB100-64932]

Immunohistochemistry-Frozen: Fibroblast Antibody (ER-TR7) [NB100-64932] - Fibroblast Antibody (ER-TR7) IHC on Mouse lymph node, frozen section, 20x. Primary antibody diluted 1:500. This image was submitted via customer Review.

Applications for Fibroblast Antibody (ER-TR7) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:300-1:500. Use reported in scientific literature (PMID 22042977)

Immunohistochemistry-Frozen

1:500

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 35794106)
Application Notes
Membrane permeabilisation is required for Flow Cytometry.

Reviewed Applications

Read 1 review rated 4 using NB100-64932 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibroblast

Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts. This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.

Alternate Names

reticular fibroblast

Additional Fibroblast Products

Product Documents for Fibroblast Antibody (ER-TR7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Fibroblast Antibody (ER-TR7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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