From your experience with NB100-1845, do you know if it's fibroblast specific? Has this antibody been tested on vascular smooth muscle cells, endothelial cells or dermal/skin fibroblasts?

The antibody recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. It does not react with vascular smooth muscle cells.

I am currently in search of an antibody for staining of human dermal fibroblasts. Can you recommend me an antibody for this? Is there an antibody that specifically stains only fibroblasts and not mesenchymal stem cells?

We sell a number of antibodies which are suitable for staining fibroblasts. You can see these at the following links: NB100-64932, NB110-15846 and NB100-1845. NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. NB110-15846 is specific to beta subunit of prolyl 4-hydroxylase. It reacts with fibroblasts and myoepithelial cells, and does not stain lymphocytes, monocytes, dendritic cells or granulocytes. NB100-1845 recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. It does not react with vascular smooth muscle cells. Unfortunately, we do not have any data regarding the staining of mesenchymal stem cells with any of these antibodies in our QC lab.

Fibroblast Surface Protein Antibody (1B10)

Name: Fibroblast Surface Protein Antibody (1B10)
Brand: Novus Biologicals
SKU: NB100-1845
Rating: 3 (2 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1845

(2)

Citations (6)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Complement-dependent Cytotoxicity, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 1B10

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Fibroblast Surface Protein Antibodies »

Product Specifications

Immunogen

cultured human thymic fibroblasts

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33296650)

Localization

Cell Membrane and Lysosome

Specificity

This fibroblast antigen has been demonstrated on human synovial, mammary, foreskin and thymic fibroblasts and in malignant fibrosarcoma tissue. The antigen is both cell membrane and lysosome associated.

Marker

Fibroblast Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Fibroblast Surface Protein Antibody (1B10)

Immunocytochemistry/ Immunofluorescence: Fibroblast Surface Protein Antibody (1B10) [NB100-1845]

Immunocytochemistry/Immunofluorescence: Fibroblast Surface Protein Antibody (1B10) [NB100-1845] - Hs68 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 Anti-Fibroblast Surface Protein antibody, Mouse monoclonal, Clone: 1B10. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Applications for Fibroblast Surface Protein Antibody (1B10)

Application

Recommended Usage

Complement-dependent Cytotoxicity

1:500

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Reviewed Applications

Read 2 reviews rated 3 using NB100-1845 in the following applications:

Immunohistochemistry (1 Review)
Immunohistochemistry-Paraffin (1 Review)

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

The product is provided as the immunoglobulin fraction of mouse ascites fluid in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA.

Preservative

0.9% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibroblast Surface Protein

Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells. It is also useful for flow cytometry, immunohistochemistry and immunoblotting.

Additional Fibroblast Surface Protein Products

Product Documents for Fibroblast Surface Protein Antibody (1B10)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Fibroblast Surface Protein Antibody (1B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

Fibroblast Surface Protein Antibody (1B10)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1845

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all Fibroblast Surface Protein Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Specificity

Marker

Clonality

Host

Isotype

Scientific Data Images for Fibroblast Surface Protein Antibody (1B10)

Immunocytochemistry/ Immunofluorescence: Fibroblast Surface Protein Antibody (1B10) [NB100-1845]

Applications for Fibroblast Surface Protein Antibody (1B10)

Complement-dependent Cytotoxicity

Flow Cytometry

Immunohistochemistry

Immunohistochemistry-Frozen

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Fibroblast Surface Protein

Additional Fibroblast Surface Protein Products

Product Documents for Fibroblast Surface Protein Antibody (1B10)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Fibroblast Surface Protein Antibody (1B10)