Fibronectin Antibody (2F4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22113

Novus Biologicals, part of Bio-Techne
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NBP2-22113
NBP2-22113SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, Hydrolysis Assay, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F4

Format

BSA Free

Concentration

1.0 mg/ml

View all Fibronectin Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Fibronectin expressed in E. coli. [UniProt# P02751]

Marker

Mesenchymal Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

262.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fibronectin Antibody (2F4) - BSA Free

Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and permeabilized using PBS with 0.1% Triton X-100 before incubating them with Fibronectin antibody or the mouse IgG1 isotype control antibody. DyLight 488 conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody was used at 1: 200 dilution to detect the antigen-primary antibody complexes and the cells were counterstained with DAPI for labeling the nuclei. This Fibronectin antibody generated the expected punctate cytoplasmic-membranous staining and the signal was localized more to the inter-cellular spaces in clustered cells.
Western Blot: Fibronectin Antibody (2F4)BSA Free [NBP2-22113]

Western Blot: Fibronectin Antibody (2F4)BSA Free [NBP2-22113]

Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa (1) and HepG2 (2) whole cell lysates using Fibronectin antibody (2F4) at 2 ug/mL.
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - BSA Free [NBP2-22113]

Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded stomach cancer tissues using Fibronectin mouse mAb with DAB staining.
View 5 more images

Applications for Fibronectin Antibody (2F4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200 - 11:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibronectin

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Entrez Gene IDs

2335 (Human)

Gene Symbol

FN1

UniProt

P02751

Additional Fibronectin Products

Product Documents for Fibronectin Antibody (2F4) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fibronectin Antibody (2F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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