Fibulin 5/DANCE Antibody (2424)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76929

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG Clone # 2424

Concentration

1 mg/ml

View all Fibulin 5/DANCE Antibodies »

Product Summary for Fibulin 5/DANCE Antibody (2424)

Immunogen

Recombinant protein within Human Fibulin 5/DANCE aa 203-378 / 448. (SwissProt: Q9UBX5 Human; SwissProt: Q9WVH9 Mouse; SwissProt: Q9WVH8 Rat)

Localization

Extracellular matrix. Secreted.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fibulin 5/DANCE Antibody (2424)

Western Blot: Fibulin 5/DANCE Antibody (2424) [NBP2-76929]

Western Blot: Fibulin 5/DANCE Antibody (2424) [NBP2-76929]

Western Blot: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: Fibulin 5/DANCE Antibody (2424) [NBP2-76929]

Immunocytochemistry/ Immunofluorescence: Fibulin 5/DANCE Antibody (2424) [NBP2-76929]

Immunocytochemistry/Immunofluorescence: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - ICC staining Fibulin 5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry: Fibulin 5/DANCE Antibody (2424) [NBP2-76929]

Immunohistochemistry: Fibulin 5/DANCE Antibody (2424) [NBP2-76929]

Immunohistochemistry: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Mouse ovary tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.
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Applications for Fibulin 5/DANCE Antibody (2424)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibulin 5/DANCE

DANCE is a secreted glycoprotein with six EGF-like domains. DANCE promotes endothelial cell adhesion through interactions with integrins. It is expressed in developing arteries and neural crest and in adult sites of vascular remodeling. Defects in DANCE function are associated with connective tissue disorders and macular degeneration.

Alternate Names

DANCE, EVEC, FBLN5, UP50

Gene Symbol

FBLN5

Additional Fibulin 5/DANCE Products

Product Documents for Fibulin 5/DANCE Antibody (2424)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fibulin 5/DANCE Antibody (2424)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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