Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FIP1L1 Antibodies »

Product Summary for FIP1L1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human factor interacting with PAP using the numbering given in entry NP_112179.2 (GeneID 81608).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FIP1L1 Antibody

Western Blot: FIP1L1 Antibody [NB100-74588]

Western Blot: FIP1L1 Antibody [NB100-74588]

Western Blot: FIP1L1 Antibody [NB100-74588] - Detection of Human FIP1 on HeLa whole cell lysate using NB100-74588. FIP1 was also immunoprecipitated by rabbit anti-FIP1 antibody NB100-74589.
Immunohistochemistry: FIP1L1 Antibody [NB100-74588]

Immunohistochemistry: FIP1L1 Antibody [NB100-74588]

Immunohistochemistry: FIP1L1 Antibody [NB100-74588] - Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti-FIP1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for FIP1L1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FIP1L1

FIP1 was originally identified as Rhe (rearranged in hypereosinophilia) and part of a fusion protein encoded by a genetic rearrangement between the N-terminal region of FIP1 (Rhe) and the C-terminal region of the platelet-derived growth factor receptor alpha (PDGFRalpha). The fusion protein exhibits aberrant tyrosine kinase activity and is associated with hypereosinophilic syndrome (HES); a rare hematologic disorder characterized by an overproduction of eosinophils that results in organ damage. FIP1 was also identified as FIP1-like 1 in a database search for a human homolog of the yeast Fip1p (factor interacting with PAP), a subunit of the yeast 3'-end processing complex, CPF (cleavage and polyadenylation factor). Human FIP1 was similarly found to interact with PAP and CPSF160 as part of the human CPSF (cleavage and polyadenylation specificity factor) complex. In association with CPSF160, FIP1 is able to recognize U-rich elements on pre-mRNA and stimulate PAP activity.

Alternate Names

DKFZp586K0717, EC 1.1.1.86, EC 4.2.1.33, Factor interacting with PAP, FIP1, FIP1 like 1 (S. cerevisiae), FIP1-like 1 protein, FLJ33619, hFip1, pre-mRNA 3'-end-processing factor FIP1, Rearranged in hypereosinophilia, RHE

Entrez Gene IDs

81608 (Human)

Gene Symbol

FIP1L1

UniProt

NP_112179.2 (Human)

Additional FIP1L1 Products

Product Documents for FIP1L1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FIP1L1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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