FKBP38 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-85535

Novus Biologicals, part of Bio-Techne
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NB110-85535
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

This protein A purified FKBP38 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP38 protein. (Uniprot: Q14318)

Reactivity Notes

A BLAST analysis was used to suggest partial cross-reactivity with FKBP38 from mouse and rat sources based on ~93% homology with the immunizing sequence. Cross-reactivity with FKBP38 from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for FKBP38 Antibody

Western Blot: FKBP38 Antibody [NB110-85535]

Western Blot: FKBP38 Antibody [NB110-85535]

Western Blot: FKBP38 Antibody [NB110-85535] - Analysis of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4 C, overnight.
Western Blot: FKBP38 Antibody [NB110-85535]

Western Blot: FKBP38 Antibody [NB110-85535]

Western Blot: FKBP38 Antibody [NB110-85535] - protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4C, overnight.

Applications for FKBP38 Antibody

Application
Recommended Usage

ELISA

1:25000

Western Blot

1:500-1:2000
Application Notes
This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 64 kDa in size corresponding to FKBP8 by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: FKBP38

FKBP8 (also known as FK506-binding protein 38) is a member of the immunophilin family that has been implicated to play an important role in apoptosis through its involvement in the mechanism that targets Bcl-2 and Bcl-xL to the outer mitochondrial membrane (OMM). Suppression of endogenous FKBP8 by RNAi or transfection of a mutant FKBP8 missing the transmembrane domain necessary for mitochondrial insertion, resulted in the translocation of Bcl-2 and Bcl-xL from the OMM to the cytosol. It has also been suggested that FKBP8 may play a role in the cross-talk between the Bcl-2 and the Calcineurin/NF-AT apoptosis signaling pathways and in cell size regulation through interactions with tuberous sclerosis genes.

Long Name

38 kDa FK506 Binding Protein

Alternate Names

FKBP8

Entrez Gene IDs

23770 (Human); 14232 (Mouse); 290652 (Rat)

Gene Symbol

FKBP8

UniProt

Q14318

Additional FKBP38 Products

Product Documents for FKBP38 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FKBP38 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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