FKBP38 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77909
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This protein A purified FKBP38 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP38 protein. (Uniprot: Q14318)
Reactivity Notes
A BLAST analysis was used to suggest partial cross-reactivity with FKBP38 from mouse and rat sources based on ~93% homology with the immunizing sequence. Cross-reactivity with FKBP38 from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads
Store vial at -20C or below prior to opening. This vial contains a relatively low volume of reagent (25 ul). To minimize loss of volume dilute 1:10 by adding 225 ul of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20C or below after dilution. Avoid cycles of freezing and thawing.
Store vial at -20C or below prior to opening. This vial contains a relatively low volume of reagent (25 ul). To minimize loss of volume dilute 1:10 by adding 225 ul of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20C or below after dilution. Avoid cycles of freezing and thawing.
Scientific Data Images for FKBP38 Antibody
Western Blot: FKBP38 Antibody [NBP1-77909]
Western Blot: FKBP38 Antibody [NBP1-77909] - Shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.FKBP38 Antibody
Western blot using protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4 C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.Applications for FKBP38 Antibody
Application
Recommended Usage
ELISA
1:25000
Western Blot
1:1000
Application Notes
This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 64 kDa in size corresponding to FKBP8 by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FKBP38
Long Name
38 kDa FK506 Binding Protein
Alternate Names
FKBP8
Gene Symbol
FKBP8
UniProt
Additional FKBP38 Products
Product Documents for FKBP38 Antibody
Product Specific Notices for FKBP38 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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