Skip to main content

FKBP38 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77909

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP1-77909

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This protein A purified FKBP38 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP38 protein. (Uniprot: Q14318)

Reactivity Notes

A BLAST analysis was used to suggest partial cross-reactivity with FKBP38 from mouse and rat sources based on ~93% homology with the immunizing sequence. Cross-reactivity with FKBP38 from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads

Store vial at -20C or below prior to opening. This vial contains a relatively low volume of reagent (25 ul). To minimize loss of volume dilute 1:10 by adding 225 ul of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20C or below after dilution. Avoid cycles of freezing and thawing.

Scientific Data Images for FKBP38 Antibody

Western Blot: FKBP38 Antibody [NBP1-77909]

Western Blot: FKBP38 Antibody [NBP1-77909]

Western Blot: FKBP38 Antibody [NBP1-77909] - Shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.
FKBP38 Antibody

FKBP38 Antibody

Western blot using protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4 C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.

Applications for FKBP38 Antibody

Application
Recommended Usage

ELISA

1:25000

Western Blot

1:1000
Application Notes
This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 64 kDa in size corresponding to FKBP8 by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FKBP38

FKBP8 (also known as FK506-binding protein 38) is a member of the immunophilin family that has been implicated to play an important role in apoptosis through its involvement in the mechanism that targets Bcl-2 and Bcl-xL to the outer mitochondrial membrane (OMM). Suppression of endogenous FKBP8 by RNAi or transfection of a mutant FKBP8 missing the transmembrane domain necessary for mitochondrial insertion, resulted in the translocation of Bcl-2 and Bcl-xL from the OMM to the cytosol.

Long Name

38 kDa FK506 Binding Protein

Alternate Names

FKBP8

Gene Symbol

FKBP8

Additional FKBP38 Products

Product Documents for FKBP38 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FKBP38 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...