Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Hamster

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # Hi52C

Concentration

1 mg/ml

View all FKBP52 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the residues of human FKBP52

Localization

Cytoplasm , Nucleus

Specificity

Detects approx 52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for FKBP52/FKBP4 Antibody (Hi52C)

Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874]

Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874]

Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Western Blot analysis of Human Cell lysates showing detection of FKBP52/FKBP4 protein using Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody, Clone Hi52C (NB110-96874). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody (NB110-96874) at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874]

Immunocytochemistry/ Immunofluorescence: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874]

Immunocytochemistry/Immunofluorescence: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody, Clone Hi52C (NB110-96874). Tissue: MCF-7 cells (metastatic mammary gland/breast cell line). Species: Human. Primary Antibody: Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody (NB110-96874) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Tom Ratajczak, Univ. of W. Australia.
Immunohistochemistry: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874]

Immunohistochemistry: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874]

Immunohistochemistry: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Immunohistochemistry analysis using Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody, Clone Hi52C (NB110-96874). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FKBP52/FKBP4 Monoclonal Antibody (NB110-96874) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
View 1 more images

Applications for FKBP52/FKBP4 Antibody (Hi52C)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

1:250

Immunoprecipitation

5ug

Western Blot

1:2000
Application Notes
0.5 ug/ml was sufficient for detection of FKBP52 in 20 ug total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.FKBP52 Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FKBP52

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin Abinding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share approx. 75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90- binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors (5).

Long Name

52 kDa FK506 Binding Protein

Alternate Names

FKBP4, FKBP59, HBI, Hsp56

Entrez Gene IDs

2288 (Human); 14228 (Mouse); 260321 (Rat)

Gene Symbol

FKBP4

UniProt

Q02790, NP_002005.1 (Human)

Additional FKBP52 Products

Product Documents for FKBP52/FKBP4 Antibody (Hi52C)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FKBP52/FKBP4 Antibody (Hi52C)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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