FLASH Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-219

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all FLASH Antibodies »

Product Specifications

Immunogen

C-terminus of the FLASH protein (proprietary peptide).

Reactivity Notes

Human. Other species have not been tested.

Localization

Cytoplasmic and nuclear

Specificity

Reacts with the C terminus of the FLASH protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

212 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FLASH Antibody - BSA Free

Western Blot: FLASH AntibodyBSA Free [NB500-219]

Western Blot: FLASH AntibodyBSA Free [NB500-219]

Western Blot: FLASH Antibody [NB500-219] - WB analysis of FLASH in lysate of NIH3T3 cells with NB500-219 at a dilution of 1:500.

Applications for FLASH Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 -1:1000
Application Notes
Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FLASH

Fas is a cell-surface receptor molecule that relays apoptotic signals into cells. When Fas is activated by the binding of its ligand, the proteolytic protein caspase-8 is recruited to a signaling complex known as DISC by binding to a Fas-associated adapter protein. A new protein, FLASH, contains a motif with oligomerizing activity whose sequence is similar to that of the Caenorhabditis elegans protein CED-4, and another domain (DRD domain) that interacts with a death-effector domain in caspase-8 or in the adapter protein. FLASH is likely a component of the DISC signaling complex and is necessary for the activation of caspase-8 in Fas-mediated apoptosis.

Alternate Names

CASP8 associated protein 2, caspase 8 associated protein 2, CED-4, FLASHKIAA1315CASP8-associated protein 2, FLICE associated huge, FLICE-associated huge protein, FLJ11208, human FLASH, RIP25FLASH homolog RIP25

Entrez Gene IDs

9994 (Human)

Gene Symbol

CASP8AP2

Additional FLASH Products

Product Documents for FLASH Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FLASH Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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