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FLI1 Antibody (FLI1/4371R) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21020

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # FLI1/4371R

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human FLI1 protein

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: THP1 or Raji cells. Human tonsil, Ewing s sarcoma (EWS) or angiosarcoma.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for FLI1 Antibody (FLI1/4371R) - BSA Free

FLI1 Antibody (FLI1/4371R) - BSA Free

FLI1 Antibody (FLI1/4371R)

Analysis of Protein Array containing more than 19,000 full-length human proteins using Fli1 Recombinant Rabbit Monoclonal Antibody (FLI1/4371R).
FLI1 Antibody (FLI1/4371R) - BSA Free

FLI1 Antibody (FLI1/4371R)

Formalin-fixed, paraffin-embedded human Ewing's Sarcoma (EWS) stained with FLI1 antibody (FLI1/4371R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
FLI1 Antibody (FLI1/4371R) - BSA Free

FLI1 Antibody (FLI1/4371R)

Formalin-fixed, paraffin-embedded human Ewing's Sarcoma (EWS) stained with FLI1 antibody (FLI1/4371R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for FLI1 Antibody (FLI1/4371R) - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10mM PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: FLI1

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2 Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

Long Name

Friend Leukemia Integration 1

Alternate Names

ERGB, SIC-1

Gene Symbol

FLI1

Additional FLI1 Products

Product Documents for FLI1 Antibody (FLI1/4371R) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FLI1 Antibody (FLI1/4371R) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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