Flightless I Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32341
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human Flightless I aa 1100-1269 / 1269. (Uniprot: Q13045)
Localization
Cytoskeleton, centrosome, nucleus, focal adhesion.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Flightless I Antibody
Western Blot: Flightless I Antibody [NBP3-32341]
Western Blot: Flightless I Antibody [NBP3-32341] - Western blot analysis of Flightless I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32341, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control:
Lane 1: Mouse lung tissue lysate
Lane 2: MCF-7 cell lysate
Immunohistochemistry: Flightless I Antibody [NBP3-32341]
Immunohistochemistry: Flightless I Antibody [NBP3-32341] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Flightless I antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32341, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Applications for Flightless I Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:500
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
1*TBS (pH7.4), 0.2% BSA and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Flightless I
Alternate Names
FLI, Fli1, flightless I homolog (Drosophila), FLILflightless I (Drosophila) homolog, MGC39265, protein flightless-1 homolog
Gene Symbol
FLII
Additional Flightless I Products
Product Documents for Flightless I Antibody
Product Specific Notices for Flightless I Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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