Flotillin-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1043
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-SISQVNHKPLRTA corresponding to C-Terminus according to NP_005794.1.
Localization
Cell Membrane
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Flotillin-1 Antibody
![Western Blot: Flotillin-1 Antibody [NB100-1043]](https://resources.bio-techne.com/images/products/Flotillin-1-Antibody-Western-Blot-NB100-1043-img0001.jpg "Western Blot: Flotillin-1 Antibody [NB100-1043]")
Western Blot: Flotillin-1 Antibody [NB100-1043]
Western Blot: Flotillin-1 Antibody [NB100-1043] - Staining of K562 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by Chemiluminescence.![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043]](https://resources.bio-techne.com/images/products/Flotillin-1-Antibody-Immunohistochemistry-Paraffin-NB100-1043-img0003.jpg "Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043]")
Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043]
Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043]](https://resources.bio-techne.com/images/products/Flotillin-1-Antibody-Immunohistochemistry-Paraffin-NB100-1043-img0002.jpg "Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043]")
Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043]
Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for Flotillin-1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5 ug/mL
Peptide ELISA
Detection limit 1:32000
Western Blot
0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx 48 kDa band observed in lysates of cell line K562 and in preliminary testing of cell lysate MCF7 (calculated MW of 47.4 kDa according to NP_005794.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Flotillin-1
Alternate Names
flotillin 1, flotillin-1, integral membrane component of caveolae
Gene Symbol
FLOT1
UniProt
Additional Flotillin-1 Products
Product Documents for Flotillin-1 Antibody
Product Specific Notices for Flotillin-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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