Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Chimpanzee (100%), Orangutan (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Flotillin-1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 377 and 427 of human Flotillin 1 using the numbering given in entry NP_005794.1 (GeneID 10211).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Flotillin-1 Antibody

Western Blot: Flotillin-1 Antibody [NBP1-79022]

Western Blot: Flotillin-1 Antibody [NBP1-79022]

Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.
Simple Western: Flotillin-1 Antibody [NBP1-79022]

Simple Western: Flotillin-1 Antibody [NBP1-79022]

Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Flotillin-1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE

Reviewed Applications

Read 1 review rated 5 using NBP1-79022 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Flotillin-1

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

Alternate Names

flotillin 1, flotillin-1, integral membrane component of caveolae

Gene Symbol

FLOT1

UniProt

NP_005794.1 (Human)

Additional Flotillin-1 Products

Product Documents for Flotillin-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Flotillin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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