Flotillin-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16508
Key Product Details
Species Reactivity
Validated:
Human, Hamster
Predicted:
Bovine (100%), Chimpanzee (100%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin-1. The exact sequence is proprietary.
Localization
Cell membrane; Peripheral membrane protein; Membrane; caveola; Peripheral membrane protein; Melanosome
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Flotillin-1 Antibody
![Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508]](https://resources.bio-techne.com/images/products/Flotillin-1-Antibody-Immunoprecipitation-NBP2-16508-img0003.jpg "Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508]")
Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508]
Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508] - IP samples: HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Western Blot: Flotillin-1 Antibody [NBP2-16508] -
Western Blot: Flotillin-1 Antibody [NBP2-16508] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for Flotillin-1 Antibody
Application
Recommended Usage
Immunoprecipitation
1:100-1:500
Simple Western
reported by internal validation
Western Blot
1:500-1:3000
Application Notes
In Simple Western internal validation: Extracted HER2+ Breast Cancer Donor FFPE and Healthy Donor FFPE as samples; separated by size; antibody dilution of 1:20; matrix was 12-230 kDa; detected by Chemiluminescence.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7)
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Flotillin-1
Alternate Names
flotillin 1, flotillin-1, integral membrane component of caveolae
Gene Symbol
FLOT1
UniProt
Additional Flotillin-1 Products
Product Documents for Flotillin-1 Antibody
Product Specific Notices for Flotillin-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.