Flotillin 2 Antibody (PSH03-41)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32343
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat, Monkey
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH03-41
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human Flotillin 2 aa 1-428 / 428. (Uniprot: Q14254)
Localization
Cell membrane, Membrane, caveola, Endosome, Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Flotillin 2 Antibody (PSH03-41)
Immunohistochemistry: Flotillin 2 Antibody (PSH03-41) [NBP3-32343]
Immunohistochemistry: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/2,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32343) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody (PSH03-41) [NBP3-32343]
Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Immunocytochemistry analysis of HeLa cells labeling Flotillin 2 with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/100 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
Flow Cytometry: Flotillin 2 Antibody (PSH03-41) [NBP3-32343]
Flow Cytometry: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Flow cytometric analysis of HeLa cells labeling Flotillin 2.Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32343, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Applications for Flotillin 2 Antibody (PSH03-41)
Application
Recommended Usage
Flow Cytometry
1:1000
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry-Paraffin
1:500-1:2000
Western Blot
1:2000-1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Flotillin 2
Alternate Names
Epidermal surface antigen, ESA1Membrane component chromosome 17 surface marker 1, ESAchromosome 17, surface marker 1 (35kD protein identified bymonoclonal ECS-1), flotillin 2
Gene Symbol
FLOT2
Additional Flotillin 2 Products
Product Documents for Flotillin 2 Antibody (PSH03-41)
Product Specific Notices for Flotillin 2 Antibody (PSH03-41)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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