Flotillin 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30881
![Western Blot: Flotillin 2 Antibody [NBP1-30881]](https://resources.bio-techne.com/images/products/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0008.jpg "Western Blot: Flotillin 2 Antibody [NBP1-30881]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Block/Neutralize, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 2. The exact sequence is proprietary.
Localization
Cell membrane, Peripheral membrane protein, Membrane, caveola, Peripheral membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Flotillin 2 Antibody
![Western Blot: Flotillin 2 Antibody [NBP1-30881]](https://resources.bio-techne.com/images/products/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0013.jpg "Western Blot: Flotillin 2 Antibody [NBP1-30881]")
Western Blot: Flotillin 2 Antibody [NBP1-30881]
Western Blot: Flotillin 2 Antibody [NBP1-30881] - 293T whole cell extracts and 293T exosome extract (2.6 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (NBP1-30881). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody [NBP1-30881]](https://resources.bio-techne.com/images/products/Flotillin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30881-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody [NBP1-30881]")
Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody [NBP1-30881]
Immunocytochemistry/Immunofluorescence: Flotillin 2 Antibody [NBP1-30881] - Analysis of paraformaldehyde-fixed HeLaS3, using antibody at 1:100 dilution.![Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881]](https://resources.bio-techne.com/images/products/Flotillin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30881-img0006.jpg "Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881]")
Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881]
Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881] - Human lung cancer, using Flotillin-2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: Flotillin 2 Antibody [NBP1-30881]](https://resources.bio-techne.com/images/products/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0012.jpg "Western Blot: Flotillin 2 Antibody [NBP1-30881]")
![Immunoprecipitation: Flotillin 2 Antibody [NBP1-30881]](https://resources.bio-techne.com/images/products/Flotillin-2-Antibody-Immunoprecipitation-NBP1-30881-img0010.jpg "Immunoprecipitation: Flotillin 2 Antibody [NBP1-30881]")
Applications for Flotillin 2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 5 using NBP1-30881 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Flotillin 2
Alternate Names
Epidermal surface antigen, ESA1Membrane component chromosome 17 surface marker 1, ESAchromosome 17, surface marker 1 (35kD protein identified bymonoclonal ECS-1), flotillin 2
Entrez Gene IDs
2319 (Human)
Gene Symbol
FLOT2
UniProt
Additional Flotillin 2 Products
Product Documents for Flotillin 2 Antibody
Product Specific Notices for Flotillin 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.