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Flotillin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98723

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Flotillin 2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Flotillin 2 Antibody

Western Blot: Flotillin 2 Antibody [NBP2-98723]

Western Blot: Flotillin 2 Antibody [NBP2-98723]

Western Blot: Flotillin 2 Antibody [NBP2-98723] - Anti-Flotillin 2 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 50 kDa
Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody [NBP2-98723]

Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody [NBP2-98723]

Immunocytochemistry/Immunofluorescence: Flotillin 2 Antibody [NBP2-98723] - Immunofluorescence staining of Flotillin 2 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human Flotillin 2 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Applications for Flotillin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1500-1:50000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Flotillin 2

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq]

Alternate Names

Epidermal surface antigen, ESA1Membrane component chromosome 17 surface marker 1, ESAchromosome 17, surface marker 1 (35kD protein identified bymonoclonal ECS-1), flotillin 2

Gene Symbol

FLOT2

Additional Flotillin 2 Products

Product Documents for Flotillin 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Flotillin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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