FMO3 Antibody (6Q10X9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15688

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15688-100ul
NBP3-15688-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6Q10X9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FMO3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human FMO3 (P31513). KPNVKEFTETSAIFEDGTIFEGIDCVIFATGYSFAYPFLDESIIKSRNNEIILFKGVFPPLLEKSTIAVIGFVQSLGAAIPTVDLQSRWAAQVIKGTCTLP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FMO3 Antibody (6Q10X9)

Western Blot: FMO3 Antibody (6Q10X9) [NBP3-15688]

Western Blot: FMO3 Antibody (6Q10X9) [NBP3-15688]

Western Blot: FMO3 Antibody (6Q10X9) [NBP3-15688] - Western blot analysis of extracts of various cell lines, using FMO3 antibody (NBP3-15688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: FMO3 Antibody (6Q10X9) [NBP3-15688]

Western Blot: FMO3 Antibody (6Q10X9) [NBP3-15688]

Western Blot: FMO3 Antibody (6Q10X9) [NBP3-15688] - Western blot analysis of extracts of various cell lines, using FMO3 antibody (NBP3-15688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
FMO3 Antibody (6Q10X9)

Immunocytochemistry/ Immunofluorescence: FMO3 Antibody (6Q10X9) [NBP3-15688] -

Immunocytochemistry/ Immunofluorescence: FMO3 Antibody (6Q10X9) [NBP3-15688] - Confocal imaging of paraffin-embedded Human liver tissue using FMO3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
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Applications for FMO3 Antibody (6Q10X9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FMO3

The mammalian flavin-containing monooxygenases (FMO; EC 1.14.13.8) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. These enzymes catalyze the NADPH-dependent oxidative metabolism of many drugs, pesticides, and other foreign compounds. Their substrates are soft nucleophiles with an electron-rich center, typically a nitrogen, sulfur or phosphorus-containing functional group, as the site for oxidative attack by the enzyme (Ziegler, 1990 [PubMed 2203193]; Hines et al., 1994 [PubMed 8128486]).[supplied by OMIM]

Alternate Names

dimethylaniline monooxygenase [N-oxide-forming] 3, Dimethylaniline oxidase 3, dJ127D3.1, EC 1.14.13.8, flavin containing monooxygenase 3, FMO 3, FMO form 2, FMO II, FMOII, Hepatic flavin-containing monooxygenase 3, hepatic flavin-containing monooxygenase-3, MGC34400, TMAU

Gene Symbol

FMO3

Additional FMO3 Products

Product Documents for FMO3 Antibody (6Q10X9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FMO3 Antibody (6Q10X9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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