FNTB Antibody (5H4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26575
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5H4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for FNTB Antibody (5H4)
Immunogen
A synthesized peptide derived from Human FNTB [UniProt P49356]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FNTB Antibody (5H4)
Immunoprecipitation: FNTB Antibody (5H4) [NBP3-26575] -
Immunoprecipitation: FNTB Antibody (5H4) [NBP3-26575] - Immunoprecipitating FNTB in K562 whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26575 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26575 (2ug) + K562 whole cell lysate (500ug).
Lane 3: K562 whole cell lysate (10ug).
Immunohistochemistry: FNTB Antibody (5H4) [NBP3-26575] -
Immunohistochemistry: FNTB Antibody (5H4) [NBP3-26575] - Image of FNTB Antibody (5H4) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: FNTB Antibody (5H4) [NBP3-26575] -
Western Blot: FNTB Antibody (5H4) [NBP3-26575] - Positive Western Blot detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue.All lanes: FNTB Antibody at 1: 2000.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 49, 44 kDa
Observed band size: 49 kDa
Applications for FNTB Antibody (5H4)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: FNTB
Long Name
Protein farnesyltransferase subunit beta
Alternate Names
FTase-beta
Gene Symbol
FNTB
Additional FNTB Products
Product Documents for FNTB Antibody (5H4)
Product Specific Notices for FNTB Antibody (5H4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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