Folliculin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44995

Novus Biologicals, part of Bio-Techne
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NBP1-44995
NBP1-44995SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (91%), Canine (95%), Primate (99%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Folliculin Antibodies »

Product Specifications

Immunogen

N-terminal partial recombinant human Folliculin protein [Swiss-Prot# Q8NFG4] expressed in E. coli.

Reactivity Notes

Immunogen sequence similarity to other species: Primate species (99%), Canine (95%), Rat (92%), Bovine (91%), Xenopus (87%).

Localization

Cytoplasm, Nucleus

Specificity

This antibody is expected to detect all three isoforms of Folliculin protein ( UniProt # Q8NFG4)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Folliculin Antibody

Western Blot: Folliculin Antibody [NBP1-44995]

Western Blot: Folliculin Antibody [NBP1-44995]

Western Blot: Folliculin Antibody [NBP1-44995] - Analysis of FLCN isoforms 1, 2 and 3 on HeLa whole cell lysate
Immunocytochemistry/ Immunofluorescence: Folliculin Antibody [NBP1-44995]

Immunocytochemistry/ Immunofluorescence: Folliculin Antibody [NBP1-44995]

Immunocytochemistry/Immunofluorescence: Folliculin Antibody [NBP1-44995] - Detection of FLCN (Green) in Hela cells using NBP1-44995. Nuclei (Blue) are counterstained using Hoechst 33258
Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995]

Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995]

Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. The SCC cells as well as the associated stromal cells depicted strong cytoplasmic-nuclear immunostaining of Folliculin protein. The staining was more intense in the carcinoma cells compared to the tumor stroma [Magnification 10X]
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Applications for Folliculin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000
Application Notes
This Folliculin antibody is useful for Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. In WB, bands are seen at 67 kDa, 37 kDa and 26 kDa reporesenting isoforms 1, 2 and 3 of FLCN respectively.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Folliculin

Folliculin (FLCN) is encoded by BHD gene (Birt-Hogg-Dube gene, altered in BHD Syndrome) and is mainly localized in the nucleus, however, has also been observed to co-localize with FLCN-interacting proteins FNIP1 and FNIP2 in the cytoplasm. Highly expressed in several tissues including skin, lung, kidney, heart, testis, stomach etc., FLCN involves in pathogenesis of proliferating epithelial fibrofolliculomas where it is overexpressed, however, its expression is significantly reduced in renal tumor tissues suggesting its tumor suppressor activity in kidneys. FLCN association with FNIP1/FNIP2 mediates its indirect interaction with AMPK subunits (PRKAA1, PRKAB1 and PRKAG1). FLCN has also been suggested to regulate phosphorylation of RPS6KB1 and implicate in energy and/or nutrient sensing through AMPK/mTOR signaling pathways. BHD knock-out mice develop cysts and renal cell tumors similar to those found in BHD patients along with low levels of phosphorylated S6 ribosomal protein. Defects in FLCN are the cause of Birt-Hogg-Dube syndrome (BHD), primary spontaneous pneumothorax (PSP), renal cell carcinoma (RCC).

Alternate Names

BHD skin lesion fibrofolliculoma protein, BHDFLCL, Birt-Hogg-Dube syndrome protein, DKFZp547A118, FLJ45004, FLJ99377, folliculin, MGC17998, MGC23445

Entrez Gene IDs

201163 (Human); 216805 (Mouse)

Gene Symbol

FLCN

UniProt

Q8NFG4 (Human), Q8QZS3 (Mouse)

Additional Folliculin Products

Product Documents for Folliculin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Folliculin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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