FOX2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40588

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FOX2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human RNA Binding Motif Protein 9 using the numbering given in entry NP_055124.1 (GeneID 23543).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FOX2 Antibody

Western Blot: FOX2 Antibody [NB110-40588]

Western Blot: FOX2 Antibody [NB110-40588]

Western Blot: FOX2 Antibody [NB110-40588] - Whole cell lysate (10 ug) from NIH 3T3, TCMK-1, CT26, and C6 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: FOX2 Antibody [NB110-40588]

Immunohistochemistry-Paraffin: FOX2 Antibody [NB110-40588]

Immunohistochemistry-Paraffin: FOX2 Antibody [NB110-40588] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-RBM9 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Western Blot: FOX2 Antibody [NB110-40588]

Western Blot: FOX2 Antibody [NB110-40588]

Western Blot: FOX2 Antibody [NB110-40588] - Whole cell lysate (10 ug) from HEK293T, HeLa, RKO, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Applications for FOX2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FOX2

This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternate Names

dJ106I20.3, Fox-1 homolog B, fox-1 homologue, FOX2Fox-2, fxh, hexaribonucleotide binding protein 2, Hexaribonucleotide-binding protein 2, HRNBP2FOX-2, RBM9RTAHNRBP2, Repressor of tamoxifen transcriptional activity, RNA binding motif protein 9, RNA binding protein fox-1 homolog 2, RNA binding protein, fox-1 homolog (C. elegans) 2, RNA-binding motif protein 9, RNA-binding protein 9

Entrez Gene IDs

23543 (Human)

Gene Symbol

RBFOX2

UniProt

NP_055124.1 (Human)

Additional FOX2 Products

Product Documents for FOX2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FOX2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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