FOXI1 Antibody (PCRP-FOXL1-1F8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13886

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-FOXL1-1F8

Concentration

0.2 mg/ml

View all FOXI1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa104-258) of human FOXI1 protein (exact sequence is proprietary) (Uniprot: Q12951)

Reactivity Notes

Predicted to react in Rat.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14224)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.

Theoretical molecular weight: 36-41kDa

Scientific Data Images for FOXI1 Antibody (PCRP-FOXL1-1F8)

Immunocytochemistry/ Immunofluorescence: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886]

Immunocytochemistry/ Immunofluorescence: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886]

Immunocytochemistry/Immunofluorescence: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Immunofluorescence analysis of PFA-fixed HeLa cells. FOXI1 Antibody (PCRP-FOXL1-1F8) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886]

Immunohistochemistry-Paraffin: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886]

Immunohistochemistry-Paraffin: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Formalin-fixed, paraffin-embedded human prostate stained. FOXI1 antibody (PCRP-FOXL1-1F8) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886]

Flow Cytometry: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886]

Flow Cytometry: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Flow cytometric analysis of PFA-fixed HeLa cells. FOXI1 antibody (PCRP-FOXL1-1F8) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
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Applications for FOXI1 Antibody (PCRP-FOXL1-1F8)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: FOXI1

FOXI1 belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

FKHL10HFH3, forkhead box I1, forkhead box protein I1, forkhead-like 10, forkhead-related activator 6, Forkhead-related protein FKHL10, Forkhead-related transcription factor 6, FREAC-6, FREAC6FKH10, Hepatocyte nuclear factor 3 forkhead homolog 3, HFH-3, HNF-3/fork-head homolog 3, HNF-3/fork-head homolog-3, MGC34197

Gene Symbol

FOXI1

UniProt

Q12951 (Human)

Additional FOXI1 Products

Product Documents for FOXI1 Antibody (PCRP-FOXL1-1F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FOXI1 Antibody (PCRP-FOXL1-1F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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