FoxM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30961

Novus Biologicals, part of Bio-Techne
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NBP1-30961
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FoxM1 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXM1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (83%).

Localization

Nucleus

Marker

Mitosis Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FoxM1 Antibody

Western Blot: FoxM1 Antibody [NBP1-30961]

Western Blot: FoxM1 Antibody [NBP1-30961]

Western Blot: FoxM1 Antibody [NBP1-30961] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody [C3], C-term diluted at 1:5000.
Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-30961]

Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-30961]

Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-30961] - Representative images of immunoreactivity of nuclear FOXM1 in (I) benign cystadenoma, (II) borderline tumour, (III) stage I invasive cancer, (IV) stage II invasive cancer, (V) stage III invasive cancer and (VI) stage IV invasive cancer. Magnifications X400. Insets: Regions with higher magnifications of nuclear FOXM1 staining. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0113478), licensed under a CC-BY license.

Applications for FoxM1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Application Notes
IHC-P reactivity reported in (PMID: 25411964). ChIP assay- Assay dependent.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-30961 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxM1

FOXM1 belongs to a large family of Forkhead transcription factors that possesses a winged-helix DNA-binding domain called Forkhead box (FOX). It is a transcription factor critical to cell cycle progression via its role in coordinating the G2/M phase transition. FOXM1 is ubiquitously expressed in proliferating cells but is absent in differentiated cells. FOXM1 overexpression has been detected in a variety of tumor types including breast, lung, prostate, and liver cancer, and appears to be involved in tumorigenesis-associated processes such as angiogenesis and metastasis. In light of these findings, FOXM1 has recently become an attractive target for anti-cancer therapy.

Long Name

Forkhead Box M1

Alternate Names

FKHL16, HFH-11, HNF-3, INS-1, MPHOSPH2, MPP-2, PIG29, Trident

Entrez Gene IDs

2305 (Human)

Gene Symbol

FOXM1

UniProt

Q08050

Additional FoxM1 Products

Product Documents for FoxM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FoxM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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