FOXO3 [p Ser253] Antibody (1E1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26424
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1E1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for FOXO3 [p Ser253] Antibody (1E1)
Immunogen
A synthesized peptide derived from Human FOXO3 [p Ser253] [UniProt O43524]
Modification
p Ser 253
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FOXO3 [p Ser253] Antibody (1E1)
![FOXO3 [p Ser253] Antibody (1E1)](https://resources.bio-techne.com/images/products/nbp3-26424_rabbit-foxo3-p-ser-253-mab-1e1-262202415355710.jpg "Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -")
Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -
Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] - Image of FOXO3 [p Ser253] Antibody (1E1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.![FOXO3 [p Ser253] Antibody (1E1)](https://resources.bio-techne.com/images/products/nbp3-26424_rabbit-foxo3-p-ser-253-mab-1e1-2722024857334.jpg "Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -")
Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -
Immunohistochemistry: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] - Image of FOXO3 [p Ser253] Antibody (1E1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.![FOXO3 [p Ser253] Antibody (1E1)](https://resources.bio-techne.com/images/products/nbp3-26424_rabbit-foxo3-p-ser-253-mab-1e1-282202411442618.jpg "Western Blot: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -")
Western Blot: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] -
Western Blot: FOXO3 [p Ser253] Antibody (1E1) [NBP3-26424] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate.All lanes: FOXO3 [p Ser253] Antibody at 0.96ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 97 KDa
Observed band size: 97 KDa
Applications for FOXO3 [p Ser253] Antibody (1E1)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: FoxO3
Long Name
Forkhead Box O3
Alternate Names
AF6q21, FKHRL1, FKHRL1P2, FOXO3A
Gene Symbol
FOXO3
Additional FoxO3 Products
Product Documents for FOXO3 [p Ser253] Antibody (1E1)
Product Specific Notices for FOXO3 [p Ser253] Antibody (1E1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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