FOXO3 [p Ser253] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81929

Novus Biologicals, part of Bio-Techne
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NB100-81929
NB100-81929-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FoxO3 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human FOXO3 around the phosphorylation site of serine 253 (A-V-SP-M-D).

Modification

p Ser253

Specificity

Detects endogenous levels of FOXO3 only when phosphorylated at serine 253.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FOXO3 [p Ser253] Antibody

Western Blot: FOXO3 [p Ser253] Antibody [NB100-81929]

Western Blot: FOXO3 [p Ser253] Antibody [NB100-81929]

Western Blot: FOXO3 [p Ser253] Antibody [NB100-81929] - Analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (Lane 3 and 4).
Immunocytochemistry/ Immunofluorescence: FOXO3 [p Ser253] Antibody [NB100-81929]

Immunocytochemistry/ Immunofluorescence: FOXO3 [p Ser253] Antibody [NB100-81929]

Immunocytochemistry/Immunofluorescence: FOXO3 [p Ser253] Antibody [NB100-81929] - Staining of methanol-fixed HeLa cells using FKHRL1 (phospho-Ser253) antibody.
Immunohistochemistry-Paraffin: FOXO3 [p Ser253] Antibody [NB100-81929]

Immunohistochemistry-Paraffin: FOXO3 [p Ser253] Antibody [NB100-81929]

Immunohistochemistry-Paraffin: FOXO3 [p Ser253] Antibody [NB100-81929] - Analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody.
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Applications for FOXO3 [p Ser253] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FoxO3

FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Long Name

Forkhead Box O3

Alternate Names

AF6q21, FKHRL1, FKHRL1P2, FOXO3A

Entrez Gene IDs

2309 (Human); 56484 (Mouse); 294515 (Rat)

Gene Symbol

FOXO3

UniProt

O43524 (Human)

Additional FoxO3 Products

Product Documents for FOXO3 [p Ser253] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FOXO3 [p Ser253] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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