FoxP1 Antibody (JC12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65125

Novus Biologicals, part of Bio-Techne
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NB100-65125
NB100-65125SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # JC12

Format

BSA Free

Concentration

1.0 mg/ml

View all FoxP1 Antibodies »

Product Specifications

Immunogen

Human FOXP1 [Uniprot# Q9H334]

Epitope

Within the C-terminus

Localization

Nuclear, Cytoplasmic

Specificity

Does not recognize closely related molecules FOXP2, FOXP3 or FOXP4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for FoxP1 Antibody (JC12) - BSA Free

Western Blot: FoxP1 Antibody (JC12)BSA Free [NB100-65125]

Western Blot: FoxP1 Antibody (JC12)BSA Free [NB100-65125]

Western Blot: FOXP1 Antibody (JC12) [NB100-65125] - Western blot analysis of resonicated MCF7 cell lysate (A) and MCF7 cell lysate (B) using FOXP1 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: FoxP1 Antibody (JC12) - BSA Free [NB100-65125]

Immunocytochemistry/ Immunofluorescence: FoxP1 Antibody (JC12) - BSA Free [NB100-65125]

Immunocytochemistry/Immunofluorescence: FOXP1 Antibody (JC12) [NB100-65125] - FOXP1 antibody was tested at 1:25 in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - BSA Free [NB100-65125]

Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - BSA Free [NB100-65125]

Immunohistochemistry-Paraffin: FOXP1 Antibody (JC12) [NB100-65125] - IHC analysis of formalin-fixed paraffin-embedded tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The carcinoma cells developed an expected and specific strong nuclear with mild cytoplasmic immunopositivity for FOXP1 protein.
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Applications for FoxP1 Antibody (JC12) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 15161711)

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Immunoprecipitation

reported in scientific literature (PMID 17586580)

Western Blot

2ug/ml
Application Notes
Heat induced antigen retrieval with Sodium Citrate buffer pH 6.0 is recommended when using this antibody for IHC-P. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxP1

FOXP1 is a member of the Forkhead Box Protein family. These proteins are transcription factors that are characterised by a highly conserved forkhead DNA-binding domain. FOXP1 is a repressor of transcription. It forms homodimers, and also heterodimerises with FOXP2 and FOXP4, to facilitate its binding to DNA for transcriptional regulation. A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukaemia, and FOXP1 is also implicated in mental retardation with language impairment and autistic features (MRLIAF). Chromosomal translocation of FOXP1 is implicated in mucosa-associated lymphoid tissue (MALT) lymphoma.

Long Name

Forkhead Box P1

Alternate Names

QRF1

Entrez Gene IDs

27086 (Human)

Gene Symbol

FOXP1

UniProt

Q9H334 (Human)

Additional FoxP1 Products

Product Documents for FoxP1 Antibody (JC12) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FoxP1 Antibody (JC12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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