FPGT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16527

Novus Biologicals, part of Bio-Techne
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NBP2-16527
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FPGT Antibodies »

Product Summary for FPGT Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human FPGT. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FPGT Antibody

Western Blot: FPGT Antibody [NBP2-16527]

Western Blot: FPGT Antibody [NBP2-16527]

Western Blot: FPGT Antibody [NBP2-16527] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: FPGT Antibody [NBP2-16527]

Immunocytochemistry/ Immunofluorescence: FPGT Antibody [NBP2-16527]

Immunocytochemistry/Immunofluorescence: FPGT Antibody [NBP2-16527] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FPGT protein stained by FPGT antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: FPGT Antibody [NBP2-16527]

Immunohistochemistry-Paraffin: FPGT Antibody [NBP2-16527]

Immunohistochemistry-Paraffin: FPGT Antibody [NBP2-16527] - human colon carcinoma, using FPGT antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for FPGT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FPGT

L-fucose is a key sugar in glycoproteins and other complex carbohydrates since it may be involved in many of the functional roles of these macromolecules, such as in cell-cell recognition. The fucosyl donor for these fucosylated oligosaccharides is GDP-beta-L-fucose. There are two alternate pathways for the biosynthesis of GDP-fucose; the major pathway converts GDP-alpha-D-mannose to GDP-beta-L-fucose. The protein encoded by this gene participates in an alternate pathway that is present in certain mammalian tissues, such as liver and kidney, and appears to function as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. This pathway involves the phosphorylation of L-fucose to form beta-L-fucose-1-phosphate, and then condensation of the beta-L-fucose-1-phosphate with GTP by fucose-1-phosphate guanylyltransferase to form GDP-beta-L-fucose. [provided by RefSeq]

Alternate Names

fucose-1-phosphate guanyltransferase, fucose-1-phosphate guanylyltransferase, GDP-beta-L-fucose pyrophosphorylase, GDP-L-fucose diphosphorylase, GDP-L-fucose pyrophosphorylase, GFPPEC 2.7.7.30

Gene Symbol

FPGT

UniProt

O14772 (Human)

Additional FPGT Products

Product Documents for FPGT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FPGT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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