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Frizzled-10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85753

Novus Biologicals, part of Bio-Techne
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NBP1-85753
NBP1-85753-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Frizzled-10 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KTLQSWQQVCSRRLKKKSRRKPASVITSGGIYKKAQHPQKTHHGKYEIPAQSPTCV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Frizzled-10 Antibody

Immunocytochemistry/ Immunofluorescence: Frizzled-10 Antibody [NBP1-85753]

Immunocytochemistry/ Immunofluorescence: Frizzled-10 Antibody [NBP1-85753]

Immunocytochemistry/Immunofluorescence: Frizzled-10 Antibody [NBP1-85753] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Frizzled-10 Antibody [NBP1-85753]

Immunohistochemistry-Paraffin: Frizzled-10 Antibody [NBP1-85753]

Immunohistochemistry-Paraffin: Frizzled-10 Antibody [NBP1-85753] - Staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.

Applications for Frizzled-10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Frizzled-10

Frizzled-10 (FZD10) is a Frizzled Receptor that mediates Wnt signaling. It is expressed during development in embryonic limbs, neural tube, and central nervous system. FZD10 has been reported to be expressed in brain, embryo, kidney, liver, lung, pancreas, placenta, skeletal muscle, spinal cord, and testis. ESTs have been isolated from brain, ear, heart/melanocyte/uterus, and kidney libraries.

Alternate Names

CD350, Frizzled10, FZD10

Gene Symbol

FZD10

Additional Frizzled-10 Products

Product Documents for Frizzled-10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Frizzled-10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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