FRMD6 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05019
![Western Blot: FRMD6 AntibodyAzide and BSA Free [NBP3-05019]](https://resources.bio-techne.com/images/products/FRMD6-Antibody-Western-Blot-NBP3-05019-img0007.jpg "Western Blot: FRMD6 AntibodyAzide and BSA Free [NBP3-05019]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 82-183 of human FRMD6 (NP_001253975.1). KEWKKEASKVRQYEVTWGIDQFGPPMIIHFRVQYYVENGRLISDRAARYYYYWHLRKQVLHSQCVLREEAYFLLAAFALQADLGNFKRNKHYGKYFEPEAYF
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FRMD6 Antibody - Azide and BSA Free
![Western Blot: FRMD6 AntibodyAzide and BSA Free [NBP3-05019]](https://resources.bio-techne.com/images/products/FRMD6-Antibody-Western-Blot-NBP3-05019-img0006.jpg "Western Blot: FRMD6 AntibodyAzide and BSA Free [NBP3-05019]")
Western Blot: FRMD6 AntibodyAzide and BSA Free [NBP3-05019]
Western Blot: FRMD6 Antibody [NBP3-05019] - Analysis of extracts of various cell lines, using FRMD6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit![Immunocytochemistry/ Immunofluorescence: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]](https://resources.bio-techne.com/images/products/FRMD6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05019-img0002.jpg "Immunocytochemistry/ Immunofluorescence: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]")
Immunocytochemistry/ Immunofluorescence: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]
Immunocytochemistry/Immunofluorescence: FRMD6 Antibody [NBP3-05019] - Analysis of U2OS cells using FRMD6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]](https://resources.bio-techne.com/images/products/FRMD6-Antibody-Immunohistochemistry-Paraffin-NBP3-05019-img0005.jpg "Immunohistochemistry-Paraffin: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]")
Immunohistochemistry-Paraffin: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]
Immunohistochemistry-Paraffin: FRMD6 Antibody [NBP3-05019] - Mouse heart using FRMD6 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunocytochemistry/ Immunofluorescence: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]](https://resources.bio-techne.com/images/products/FRMD6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05019-img0001.jpg "Immunocytochemistry/ Immunofluorescence: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]")
![Immunohistochemistry-Paraffin: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]](https://resources.bio-techne.com/images/products/FRMD6-Antibody-Immunohistochemistry-Paraffin-NBP3-05019-img0003.jpg "Immunohistochemistry-Paraffin: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]")
![Immunohistochemistry-Paraffin: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]](https://resources.bio-techne.com/images/products/FRMD6-Antibody-Immunohistochemistry-Paraffin-NBP3-05019-img0004.jpg "Immunohistochemistry-Paraffin: FRMD6 Antibody - Azide and BSA Free [NBP3-05019]")
Applications for FRMD6 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:1000-1:4000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FRMD6
Alternate Names
FERM domain containing 6
Gene Symbol
FRMD6
Additional FRMD6 Products
Product Documents for FRMD6 Antibody - Azide and BSA Free
Product Specific Notices for FRMD6 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov