FRS2 Antibody (JE30-77)

Western Blot: FRS2 Antibody (JE30-77) [NBP3-32351]

Western Blot: FRS2 Antibody (JE30-77) [NBP3-32351] - Western blot analysis of FRS2 on different lysates with Rabbit anti-FRS2 antibody (NBP3-32351) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: NCI-H441 cell lysate
Lane 5: MCF7 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 57 kDa
Observed band size: 70 kDa

Exposure time: 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32351) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: FRS2 Antibody (JE30-77) [NBP3-32351]

Flow Cytometry: FRS2 Antibody (JE30-77) [NBP3-32351] - Flow cytometric analysis of HeLa cells labeling FRS2.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32351, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).