FRS2 Antibody (SR2015)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21759

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2015

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FRS2 Antibodies »

Product Summary for FRS2 Antibody (SR2015)

Immunogen

A synthesized peptide derived from human FRS2 (Uniprot #: Q8WU20)

Specificity

Detects endogenous levels of total FRS2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FRS2 Antibody (SR2015)

Western Blot: FRS2 Antibody (SR2015) [NBP3-21759] -

Western Blot: FRS2 Antibody (SR2015) [NBP3-21759] - Western blot analysis using NBP3-21759 in HeLa cell lysate.

Applications for FRS2 Antibody (SR2015)

Application
Recommended Usage

Flow Cytometry

1:20

Immunoprecipitation

1:50

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FRS2

FGFs (Fibroblast Growth Factors) bind to FGF receptors (FGFRs) monovalently, and FGF receptor dimerization and activation is mediated by multivalent interactions between heparin sulfate proteoglycans and FGF. Upon activation, receptor tyrosine kinases undergo rapid autophosphorylation on numerous tyrosine residues. Autophosphorylation sites located within the catalytic domain are crucial for stimulation of kinase activity, while autophosphorylation sites located in other regions are usually involved in the recruitment of cellular target proteins. Fibroblast growth factor or nerve growth factor (NGF) stimulation leads to tyrosine phosphorylation of the FGF Receptor Substrate 2 (FRS2) docking proteins. FRS2 is also designated SNT (Suc-1 associated neurotrophic factor target protein). Two structurally similar forms of FRS2 are: FRS2a (SNT-1) and FRS-2b (SNT-2, FRS3). FRS functions as a lipid-anchored docking protein that is tyrosine phosphorylated and recruited to FGFR upon FGF stimulation. FRS2 plays an important role in linking FGF and NGF with the Ras/MAPK signaling pathway, thus relaying information from the cell surface to the nucleus. FRS2 contains both a consensus myristylation sequence, involved in its recruitment to the cell membrane and a putative phosphotyrosine binding (PTB)domain in its amino-terminus. The interaction of FRS2 with FGFR1 occurs via the PTB domain of FRS2 binding to a 12-residue segment in the juxtamembrane region of FGFR1. FRS2a contains four binding sites for the adaptor protein Grb2 and two binding sites for the protein tyrosine phosphatase Shp2. FGF stimulation leads to phosphorylation of Shp2 on a tyrosine residue forming a complex with an additional molecule of Grb2. Grb2/Sos complexes are thus recruited directly and indirectly via Shp2 upon tyrosine phosphorylation of FRS2a in response to growth factor stimulation. It has been shown that FRS2 proteins are tyrosine phos phorylated in response to activation of the RET receptor, a tyrosine kinase that functions as the signal transducing receptor for the GDNF.

Long Name

Fibroblast Growth Factor Receptor Substrate 2

Alternate Names

FRS2alpha, SNT1

Gene Symbol

FRS2

Additional FRS2 Products

Product Documents for FRS2 Antibody (SR2015)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FRS2 Antibody (SR2015)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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