FRS2 Antibody (T5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29886

Novus Biologicals, part of Bio-Techne
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NBP2-29886
NBP2-29886-0.05mg
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # T5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FRS2 Antibodies »

Product Summary for FRS2 Antibody (T5)

Immunogen

Peptide corresponding to residues 283-297 of human FRS2/SNT-1.

Reactivity Notes

Human, Rat

Specificity

FRS2/SNT-1 in unstimulated cells and phosphorylated FRS2/SNT-1 in NGF-stimulated cellsnon-phosphorylated(68kDa) and phosphorylated(83kDa) weight may be detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

Relevant Entrez Gene Numbers: NM_006654

Scientific Data Images for FRS2 Antibody (T5)

Western Blot: FRS2 Antibody (T5) [NBP2-29886]

Western Blot: FRS2 Antibody (T5) [NBP2-29886]

Western Blot: FRS2 Antibody [NBP2-29886] - IP/Lane 1: PC12 cell llysate was incubated with non-specific IgG; Lane 2: PC12 cell lysate was incubated with Anti-FRS2/SNT-1; Lane 3: NGF stimulated PC12 cell lysate was incubated with Anti-FRS2/SNT-1; Lane 4: NGF stimulated PC12 cell lysate was incubated with non-specific IgG. Lanes 1-4 were then probed with Anti-FRS2/SNT-1 at 1ug/ml.

Applications for FRS2 Antibody (T5)

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

70mM Tris-Glycine (pH 7.4), 105mM NaCl and 30% Glycerol

Preservative

0.035% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FRS2

FGFs (Fibroblast Growth Factors) bind to FGF receptors (FGFRs) monovalently, and FGF receptor dimerization and activation is mediated by multivalent interactions between heparin sulfate proteoglycans and FGF. Upon activation, receptor tyrosine kinases undergo rapid autophosphorylation on numerous tyrosine residues. Autophosphorylation sites located within the catalytic domain are crucial for stimulation of kinase activity, while autophosphorylation sites located in other regions are usually involved in the recruitment of cellular target proteins. Fibroblast growth factor or nerve growth factor (NGF) stimulation leads to tyrosine phosphorylation of the FGF Receptor Substrate 2 (FRS2) docking proteins. FRS2 is also designated SNT (Suc-1 associated neurotrophic factor target protein). Two structurally similar forms of FRS2 are: FRS2a (SNT-1) and FRS-2b (SNT-2, FRS3). FRS functions as a lipid-anchored docking protein that is tyrosine phosphorylated and recruited to FGFR upon FGF stimulation. FRS2 plays an important role in linking FGF and NGF with the Ras/MAPK signaling pathway, thus relaying information from the cell surface to the nucleus. FRS2 contains both a consensus myristylation sequence, involved in its recruitment to the cell membrane and a putative phosphotyrosine binding (PTB)domain in its amino-terminus. The interaction of FRS2 with FGFR1 occurs via the PTB domain of FRS2 binding to a 12-residue segment in the juxtamembrane region of FGFR1. FRS2a contains four binding sites for the adaptor protein Grb2 and two binding sites for the protein tyrosine phosphatase Shp2. FGF stimulation leads to phosphorylation of Shp2 on a tyrosine residue forming a complex with an additional molecule of Grb2. Grb2/Sos complexes are thus recruited directly and indirectly via Shp2 upon tyrosine phosphorylation of FRS2a in response to growth factor stimulation. It has been shown that FRS2 proteins are tyrosine phos phorylated in response to activation of the RET receptor, a tyrosine kinase that functions as the signal transducing receptor for the GDNF.

Long Name

Fibroblast Growth Factor Receptor Substrate 2

Alternate Names

FRS2alpha, SNT1

Gene Symbol

FRS2

UniProt

Q8WU20

Additional FRS2 Products

Product Documents for FRS2 Antibody (T5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FRS2 Antibody (T5)

Relevant Entrez Gene Numbers: NM_006654

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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