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FTSJ1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26088

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for FTSJ1 Antibody - Azide and BSA Free

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human FTSJ1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FTSJ1 Antibody - Azide and BSA Free

FTSJ1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: FTSJ1 Antibody - Azide and BSA Free [NBP3-26088] -

Immunocytochemistry/ Immunofluorescence: FTSJ1 Antibody - Azide and BSA Free [NBP3-26088] - Immunofluorescence staining of FTSJ1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-26088 (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
FTSJ1 Antibody - Azide and BSA Free

Western Blot: FTSJ1 Antibody - Azide and BSA Free [NBP3-26088] -

Western Blot: FTSJ1 Antibody - Azide and BSA Free [NBP3-26088] - NBP3-26088 at 1:500 dilution. Lane A: MDA-MB453 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa

Applications for FTSJ1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry

/Immunofluorescence 1:100-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS, pH 7.0

Format

Azide and BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FTSJ1

FTSJ1 is encoded by this gene is a member of the S-adenosylmethionine-binding protein family. It is a nucleolar protein and may be involved in the processing and modification of rRNA. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Alternate Names

CDLIV, cell division protein, EC 2.1.1.-, FtsJ homolog 1 (E. coli), JM23, mental retardation, X-linked 44, mental retardation, X-linked 9, MRX44, Protein ftsJ homolog 1, putative ribosomal RNA methyltransferase 1, rRNA (uridine-2'-O-)-methyltransferase, SPB1MRX9, TRM7

Gene Symbol

FTSJ1

Additional FTSJ1 Products

Product Documents for FTSJ1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FTSJ1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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