FUBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83918

Novus Biologicals, part of Bio-Techne
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NBP1-83918
NBP1-83918-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FUBP1 Antibodies »

Product Summary for FUBP1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GGGGVNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLEDGDQPDAKKVAPQNDSFGTQLPPMHQQQRSVMTEEYKVPDGMVGFIIGRGGEQISRIQQESGCKIQIAPDSGGLPERSCML

Marker

Nucleus without Nucleoli Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FUBP1 Antibody

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining in human lymph node and skeletal muscle tissues.. Corresponding FUBP1 RNA-seq data are presented for the same tissues.
Western Blot: FUBP1 Antibody [NBP1-83918]

Western Blot: FUBP1 Antibody [NBP1-83918]

Western Blot: FUBP1 Antibody [NBP1-83918] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: FUBP1 Antibody [NBP1-83918]

Immunocytochemistry/ Immunofluorescence: FUBP1 Antibody [NBP1-83918]

Immunocytochemistry/Immunofluorescence: FUBP1 Antibody [NBP1-83918] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
View 6 more images

Applications for FUBP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FUBP1

FUBP1 encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.

Alternate Names

far upstream element (FUSE) binding protein 1, far upstream element binding protein, far upstream element-binding protein 1, FBPDNA helicase V, FUBP, FUSE-binding protein 1, hDH V

Gene Symbol

FUBP1

Additional FUBP1 Products

Product Documents for FUBP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FUBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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