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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2-25LE

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).

Specificity

The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free

Immunohistochemistry: Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free [NB500-526]

Immunohistochemistry: Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free [NB500-526]

Immunohistochemistry: Blood Group Lewis b Antibody (2-25LE) [NB500-526] - Immunohistochemistry staining of human colon adenocarcinoma (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).
Immunohistochemistry: Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free [NB500-526]

Immunohistochemistry: Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free [NB500-526]

Immunohistochemistry: Blood Group Lewis b Antibody (2-25LE) [NB500-526] - Staining of human small intestine (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).

Applications for Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

1:1000
Application Notes
Purity is > 95% (by SDS-PAGE). Heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Fucosyltransferase 3/FUT3

The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.

Alternate Names

CD174, FT3B, FucT-III, FUT3, Les, Lewis FT

Entrez Gene IDs

2525 (Human)

Gene Symbol

FUT3

UniProt

Additional Fucosyltransferase 3/FUT3 Products

Product Documents for Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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