Fucosyltransferase 3/FUT3 Antibody (SPM194) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47928

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Guinea Pig

Applications

Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM194

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Fucosyltransferase 3/FUT3 Antibodies »

Product Summary for Fucosyltransferase 3/FUT3 Antibody (SPM194) - Azide and BSA Free

Immunogen

Mucin isolated from a human ovarian cyst fluid (Uniprot: P21217)

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44597).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Fucosyltransferase 3/FUT3 Antibody (SPM194) - Azide and BSA Free

Immunohistochemistry-Paraffin: Fucosyltransferase 3/FUT3 Antibody (SPM194) - Azide and BSA Free [NBP2-47928]

Immunohistochemistry-Paraffin: Fucosyltransferase 3/FUT3 Antibody (SPM194) - Azide and BSA Free [NBP2-47928]

Immunohistochemistry-Paraffin: Fucosyltransferase 3/FUT3 Antibody (SPM194) - Azide and BSA Free [NBP2-47928] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Fucosyltransferase 3/FUT3 Antibody (SPM194).

Applications for Fucosyltransferase 3/FUT3 Antibody (SPM194) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Fucosyltransferase 3/FUT3

Because N-, O-glycans and glycolipids are frequently fucosylated at terminal sites, fucose is often found to be essential for sugar epitope and lectin ligand generation. Well-known fucose containing structures include Lewis structures and ABO blood group antigens. Lewis structures are key elements involved in leukocyte homing and extravasation process and thus are essential for lymphocyte maturation and natural defense functions. Fucose containing glycans also play essential roles in cell signaling and development. So far, more than 10 fucosyltransferases have been cloned from the human genome. FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood group antigen synthesis. FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are responsible for Lewis structure generation through their alpha 1-3 or alpha 1-4 fucosyltransferases activities. FUT3, also known as Lewis blood group fucosyltransferase, is unique by having both strong alpha 1-3 and alpha 1-4 fucosyltransferase activities. FUT3 has high homology with FUT5 and FUT6 due to gene duplication. FUT7 is exclusively responsible for biosynthesis of sialyl Lewis X epitope in leukocytes and high endothelial venule cells. FUT8 is an alpha 1-6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans. Predicted as type II transmembrane proteins and Golgi enzymes, some of the fucosyltransferases can also be found in plasma. R&D Systems rhFUTs correspond to the luminal domains.

Alternate Names

CD174, FT3B, FucT-III, FUT3, Les, Lewis FT

Gene Symbol

FUT3

Additional Fucosyltransferase 3/FUT3 Products

Product Documents for Fucosyltransferase 3/FUT3 Antibody (SPM194) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fucosyltransferase 3/FUT3 Antibody (SPM194) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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