Fumarase Antibody (OTI1F10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70733

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Fumarase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fumarase Antibody (OTI1F10) - Azide and BSA Free

Western Blot: Fumarase Antibody (OTI1F10)Azide and BSA Free [NBP2-70733]

Western Blot: Fumarase Antibody (OTI1F10)Azide and BSA Free [NBP2-70733]

Western Blot: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Fumarase.
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733]

Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733]

Immunocytochemistry/Immunofluorescence: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.
Immunohistochemistry: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733]

Immunohistochemistry: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733]

Immunohistochemistry: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.
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Applications for Fumarase Antibody (OTI1F10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fumarase

Fumarase is encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Alternate Names

EC 4.2.1.2, Fumarase, fumarate hydratase, fumarate hydratase, mitochondrial, HLRCC, LRCC, MCL, MCUL1

Gene Symbol

FH

Additional Fumarase Products

Product Documents for Fumarase Antibody (OTI1F10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fumarase Antibody (OTI1F10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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