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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F4

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant FUS2 (1-308aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for FUS2 Antibody (2F4) - BSA Free

Western Blot: FUS2 Antibody (2F4)BSA Free [NBP1-41157]

Western Blot: FUS2 Antibody (2F4)BSA Free [NBP1-41157]

Western Blot: FUS2 Antibody (2F4) - BSA Free [NBP1-41157] - Cell lysates of NIH3T3(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FUS2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: FUS2 Antibody (2F4) - BSA Free [NBP1-41157]

Immunocytochemistry/ Immunofluorescence: FUS2 Antibody (2F4) - BSA Free [NBP1-41157]

Immunocytochemistry/Immunofluorescence: FUS2 Antibody (2F4) - BSA Free [NBP1-41157] - Immunofluorescence of human A549 cells stained with Hoechst 33342 (Blue) and monoclonal anti-human FUS2 antibody (1:500) with Texas Red (red).
Flow Cytometry: FUS2 Antibody (2F4) - BSA Free [NBP1-41157]

Flow Cytometry: FUS2 Antibody (2F4) - BSA Free [NBP1-41157]

Flow Cytometry: FUS2 Antibody (2F4) - BSA Free [NBP1-41157] - Flow cytometry analysis of FUS2 in Balb/3T3 cells. The cell was stained with NBP1-41157 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Applications for FUS2 Antibody (2F4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FUS2

Vertebrate FUS2 genes, which are known to be putative tumor suppressor gene, contain several important domains such as the catalytic N-acetyltransferase (NAT) domain. NAT domain is essential enzymes involved in several sophisticated cellular processes such as N-acetylation, O-acetylatin. NAT enzymes may be involved in susceptibility to cancer including colorectal cancer because of the presence of carcinogenic heterocyclic amines in some cooked foods. FUS2 was physically localized to the cytoplasm. Also, FUS2 showed the actin dependent movement, closely related to the polarization in the budding yeast, Saccharomyces cerevisiae.

Alternate Names

FUS-2, FUS2EC 2.3.1.-, N-acetyltransferase 6, N-acetyltransferase 6 (GCN5-related), Protein fus-2, Protein fusion-2

Entrez Gene IDs

24142 (Human)

Gene Symbol

NAA80

Additional FUS2 Products

Product Documents for FUS2 Antibody (2F4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FUS2 Antibody (2F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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