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FYCO1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47266

Novus Biologicals, part of Bio-Techne
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NBP1-47266

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1428 and 1478 of human FYVE and Coiled-Coil Domain Containing 1 using the numbering given in entry NP_078789.2 (GeneID 79443).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22619176).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FYCO1 Antibody

Western Blot: FYCO1 Antibody [NBP1-47266]

Western Blot: FYCO1 Antibody [NBP1-47266]

Western Blot: FYCO1 Antibody [NBP1-47266] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FYCO1 antibody NBP1-47266 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 10 ug/mg lysate. FYCO1 was also immunoprecipitated by rabbit anti-FYCO1 antibody NBP1-47265, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for FYCO1 Antibody

Application
Recommended Usage

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FYCO1

FYCO1, Fyve and Coiled-Coil domain-containing 1, is a gene that is lost in chromosomal deletions in nearly all types of solid tumors. FYCO1 forms a complex with LC3 and Rab7 that promotes microtubule plus end-directed transport of autophagic vesicles. It is possible that FYCO1 has membrane-tethering functions as well.

Alternate Names

DKFZp779K1152, FLJ13335, FYVE and coiled-coil domain containing 1, FYVE and coiled-coil domain-containing protein 1, MGC126517, RUFY3, RUN and FYVE domain containing 3, ZFYVE7MGC126519, Zinc finger FYVE domain-containing protein 7

Entrez Gene IDs

79443 (Human)

Gene Symbol

FYCO1

UniProt

Additional FYCO1 Products

Product Documents for FYCO1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FYCO1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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