G protein alpha Antibody (7G6G5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52476

Novus Biologicals, part of Bio-Techne
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NBP2-52476
NBP2-52476-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7G6G5

Format

BSA Free

Concentration

1 mg/ml

View all G protein alpha Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human G protein alpha (AA: 42-188) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for G protein alpha Antibody (7G6G5) - BSA Free

Western Blot: G protein alpha Antibody (7G6G5)BSA Free [NBP2-52476]

Western Blot: G protein alpha Antibody (7G6G5)BSA Free [NBP2-52476]

Western Blot: G protein alpha Antibody (7G6G5) [NBP2-52476] - Analysis using GNAS mAb against human GNAS (AA: 42-188) recombinant protein. (Expected MW is 42.8 kDa)
Immunocytochemistry/ Immunofluorescence: G protein alpha Antibody (7G6G5) - BSA Free [NBP2-52476]

Immunocytochemistry/ Immunofluorescence: G protein alpha Antibody (7G6G5) - BSA Free [NBP2-52476]

Immunocytochemistry/Immunofluorescence: G protein alpha Antibody (7G6G5) [NBP2-52476] - Analysis of Hela cells using GNAS mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: G protein alpha Antibody (7G6G5) - BSA Free [NBP2-52476]

Flow Cytometry: G protein alpha Antibody (7G6G5) - BSA Free [NBP2-52476]

Flow Cytometry: G protein alpha Antibody (7G6G5) [NBP2-52476] - Analysis of MCF-7 cells using GNAS mouse mAb (green) and negative control (red).
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Applications for G protein alpha Antibody (7G6G5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G protein alpha

G protein alpha has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq]

Alternate Names

Adenylate cyclase-stimulating G alpha protein, AHO, Alternative gene product encoded by XL-exon, Extra large alphas protein, GNAS complex locus, GNAS1GSA, GNASXL, GPSAC20orf45, GSP, guanine nucleotide binding protein (G protein), alpha stimulating activitypolypeptide 1, guanine nucleotide regulatory protein, guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas, MGC33735, NESP, NESP55, neuroendocrine secretory protein, PHP1A, PHP1B, PHP1C, POH, protein ALEX, SCG6, secretogranin VI, XLalphas

Gene Symbol

GNAS

Additional G protein alpha Products

Product Documents for G protein alpha Antibody (7G6G5) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for G protein alpha Antibody (7G6G5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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