G protein alpha Antibody (A8F11-R)

Western Blot: G protein alpha Antibody (A8F11-R) [NBP3-32396]

Western Blot: G protein alpha Antibody (A8F11-R) [NBP3-32396] - Western blot analysis of G protein alpha on different lysates with Mouse anti-G protein alpha antibody (NBP3-32396) at 1/1,000 dilution.

Lane 1: NIH/3T3 cell lysate (20 ug/Lane)
Lane 2: RAW264.7 cell lysate (20 ug/Lane)
Lane 3: C6 cell lysate (20 ug/Lane)
Lane 4: Human brain tissue lysate (40 ug/Lane)
Lane 5: Mouse brain tissue lysate (40 ug/Lane)
Lane 6: Rat brain tissue lysate (40 ug/Lane)

Predicted band size: 46 kDa
Observed band size: 44 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32396) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: G protein alpha Antibody (A8F11-R) [NBP3-32396]

Immunohistochemistry: G protein alpha Antibody (A8F11-R) [NBP3-32396] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Mouse anti-G protein alpha antibody (NBP3-32396) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32396) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: G protein alpha Antibody (A8F11-R) [NBP3-32396]

Immunocytochemistry/ Immunofluorescence: G protein alpha Antibody (A8F11-R) [NBP3-32396] - Immunocytochemistry analysis of MCF7 cells labeling G protein alpha with Mouse anti-G protein alpha antibody (NBP3-32396) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-G protein alpha antibody (NBP3-32396) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.