G protein alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49874

Novus Biologicals, part of Bio-Techne
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NBP1-49874
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Electron Microscopy, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all G protein alpha Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QAARSNSDGEKATK corresponding to internal region according to NP_001070956.1, NP_001070957.1.

Epitope

QAARSNSDGEKATK - internal region

Specificity

This antibody is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for G protein alpha Antibody

Western Blot: G protein alpha Antibody [NBP1-49874]

Western Blot: G protein alpha Antibody [NBP1-49874]

Western Blot: G protein alpha Antibody [NBP1-49874] - (0.3ug/ml) staining of KNRK cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot: G protein alpha Antibody [NBP1-49874]

Western Blot: G protein alpha Antibody [NBP1-49874]

Western Blot: G protein alpha Antibody [NBP1-49874] - (0.5ug/ml) staining of Human Cerebellum (A) and (0.1ug/ml) Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for G protein alpha Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.5 ug/ml
Application Notes
WB: Approx. 45kDa band observed in Human Cerebellum, Mouse and Rat Brain lysates, and in lysates of cell line KNRK (calculated MW of 45.6kDa according to Human NP_963910.1, Mouse NP_963910.1 and Rat NP_062005.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: G protein alpha

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq]

Alternate Names

Adenylate cyclase-stimulating G alpha protein, AHO, Alternative gene product encoded by XL-exon, Extra large alphas protein, GNAS complex locus, GNAS1GSA, GNASXL, GPSAC20orf45, GSP, guanine nucleotide binding protein (G protein), alpha stimulating activitypolypeptide 1, guanine nucleotide regulatory protein, guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas, MGC33735, NESP, NESP55, neuroendocrine secretory protein, PHP1A, PHP1B, PHP1C, POH, protein ALEX, SCG6, secretogranin VI, XLalphas

Entrez Gene IDs

2778 (Human); 14683 (Mouse); 24896 (Rat)

Gene Symbol

GNAS

UniProt

NP_000507.1 (Human)

Additional G protein alpha Products

Product Documents for G protein alpha Antibody

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Certificate of Analysis

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Product Specific Notices for G protein alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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