G protein alpha Inhibitor 2 Antibody (3G11)
Novus Biologicals, part of Bio-Techne | Catalog # H00002771-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 3G11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GNAI2 (AAH12138, 1 a.a. ~ 355 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Specificity
GNAI2 - guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for G protein alpha Inhibitor 2 Antibody (3G11)
![Western Blot: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02]](https://resources.bio-techne.com/images/products/G-protein-alpha-Inhibitor-2-Antibody-3G11-Western-Blot-H00002771-M02-img0005.jpg "Western Blot: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02]")
Western Blot: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02]
Western Blot: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02] - GNAI2 monoclonal antibody (M02), clone 3G11. Analysis of GNAI2 expression in MCF-7.![Proximity Ligation Assay: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02]](https://resources.bio-techne.com/images/products/G-protein-alpha-Inhibitor-2-Antibody-3G11-Proximity-Ligation-Assay-H00002771-M02-img0003.jpg "Proximity Ligation Assay: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02]")
Proximity Ligation Assay: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02]
Proximity Ligation Assay: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02] - Analysis of protein-protein interactions between C5AR1 and GNAI2. HeLa cells were stained with anti-C5AR1 rabbit purified polyclonal 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02] - Detection limit for recombinant GST tagged GNAI2 is approximately 0.3ng/ml as a capture antibody.
Applications for G protein alpha Inhibitor 2 Antibody (3G11)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: G protein alpha Inhibitor 2
Alternate Names
alpha-2 subunit, guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 2
Entrez Gene IDs
2771 (Human)
Gene Symbol
GNAI2
OMIM
139360 (Human)
UniProt
Additional G protein alpha Inhibitor 2 Products
Product Documents for G protein alpha Inhibitor 2 Antibody (3G11)
Product Specific Notices for G protein alpha Inhibitor 2 Antibody (3G11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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