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G2A/GPR132 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-02347

Novus Biologicals, part of Bio-Techne
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NBP1-02347

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Synthetic 18 amino acid peptide from C-terminus of human G2A/GPR132.

Epitope

C-Terminus

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (89%), Marmoset (89%), Gibbon (83%), Monkey (83%).

Specificity

Human G2A/GPR132. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for G2A/GPR132 Antibody - BSA Free

Immunohistochemistry-Paraffin: G2A/GPR132 Antibody - BSA Free [NBP1-02347]

Immunohistochemistry-Paraffin: G2A/GPR132 Antibody - BSA Free [NBP1-02347]

Immunohistochemistry-Paraffin: G2A/GPR132 Antibody - BSA Free [NBP1-02347] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 11 ug/ml.

Applications for G2A/GPR132 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

12 ug/ml
Application Notes
IHC: NBP1-02347was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: G2A/GPR132

G2A belongs to the Lysophospholipid/Lysosphingolipid Receptor family and is an anti-proliferative cell cycle regulator that plays a critical role in controlling peripheral lymphocyte homeostasis. Loss of G2A expression leads to the development of a late-onset autoimmune syndrome (Le et al, 2001). Lysophosphatidylcholine (LPC) is the activating ligand for G2A.

Long Name

G2 Accumulation Protein

Alternate Names

GPR132

Gene Symbol

GPR132

UniProt

Additional G2A/GPR132 Products

Product Documents for G2A/GPR132 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for G2A/GPR132 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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