G2E3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12582
![Immunohistochemistry-Paraffin: G2E3 Antibody [NBP3-12582]](https://resources.bio-techne.com/images/products/G2E3-Antibody-Immunohistochemistry-Paraffin-NBP3-12582-img0002.jpg "Immunohistochemistry-Paraffin: G2E3 Antibody [NBP3-12582]")
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human G2E3 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for G2E3 Antibody
![Western Blot: G2E3 Antibody [NBP3-12582]](https://resources.bio-techne.com/images/products/G2E3-Antibody-Western-Blot-NBP3-12582-img0001.jpg "Western Blot: G2E3 Antibody [NBP3-12582]")
Western Blot: G2E3 Antibody [NBP3-12582]
Western Blot: G2E3 Antibody [NBP3-12582] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:80 kDaObserved band size:80 kDa![Immunocytochemistry/ Immunofluorescence: G2E3 Antibody [NBP3-12582]](https://resources.bio-techne.com/images/products/G2E3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12582-img0004.jpg "Immunocytochemistry/ Immunofluorescence: G2E3 Antibody [NBP3-12582]")
Immunocytochemistry/ Immunofluorescence: G2E3 Antibody [NBP3-12582]
Immunocytochemistry/Immunofluorescence: G2E3 Antibody [NBP3-12582] - Staining of G2E3 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human G2E3 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus and Cytoplasm.![Immunohistochemistry-Paraffin: G2E3 Antibody [NBP3-12582]](https://resources.bio-techne.com/images/products/G2E3-Antibody-Immunohistochemistry-Paraffin-NBP3-12582-img0003.jpg "Immunohistochemistry-Paraffin: G2E3 Antibody [NBP3-12582]")
Immunohistochemistry-Paraffin: G2E3 Antibody [NBP3-12582]
Immunohistochemistry-Paraffin: G2E3 Antibody [NBP3-12582] - Staining of human G2E3 in human testis with rabbit polyclonal antibody at 1:1000 dilution.Applications for G2E3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500-1:2000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: G2E3
Alternate Names
EC 6.3.2, EC 6.3.2.-, FLJ20333, G2/M phase-specific E3 ubiquitin-protein ligase, G2/M-phase specific E3 ubiquitin ligase, G2/M-phase specific E3 ubiquitin protein ligase, KIAA1333PHD finger protein 7B, PHF7B
Gene Symbol
G2E3
Additional G2E3 Products
Product Specific Notices for G2E3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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