Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all G9a/EHMT2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1160 and the C-terminus (residue 1210) of human G9A (Euchromatic Histone-Lysine N-methyltransferase 2) using the numbering given in entry NP_006700.2 (GeneID 10919).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for G9a/EHMT2 Antibody

Western Blot: G9a/EHMT2 Antibody [NB100-40825]

Western Blot: G9a/EHMT2 Antibody [NB100-40825]

Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Detection of Human G9A/EHMT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825]

Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825]

Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825]

Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825]

Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825] - Detection of human G9A/EHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 (lot NB100-40825-2) used for IP at 6 ug per reaction. G9A/EHMT2 was also immunoprecipitated by a previous lot of this antibody (NB100-40825-1) and another rabbit anti-G9A/EHMT2 antibody. For blotting immunoprecipitated G9A/EHMT2, NB100-40825 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for G9a/EHMT2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 -1:5000

Immunoprecipitation

2-10 ug/mg lysate
Application Notes
While trace amounts of G9A/EHMT2 can be immunoprecipitated by this antibody, the efficiency is very low. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 3 using NB100-40825 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: G9a/EHMT2

G9A antibody contains ankyrin repeats, suggesting involvement in intracellular protein-protein interaction. G9A is a histone methyltransferase that preferentially methylates Lys-9 of histone H3 and Lys-27 of histone H3 (in vitro). H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9A is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1.

Long Name

G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2

Alternate Names

EHMT2

Entrez Gene IDs

10919 (Human)

Gene Symbol

EHMT2

UniProt

Q96KQ7 (Human)

Additional G9a/EHMT2 Products

Product Documents for G9a/EHMT2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for G9a/EHMT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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