GABA-A R beta 1 Antibody (S96-55)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48319
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Microarray, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S96-55
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein amino acids 327-450 of mouse GABA-A-R-Beta 1
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 28197552).
Localization
Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
Specificity
Detects approx 55kDa. No cross-reactivity against GABA-A-R-Beta 2 or -Beta3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for GABA-A R beta 1 Antibody (S96-55)
![Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319]](https://resources.bio-techne.com/images/products/GABA-A-R-beta-1-Antibody-S96-55-Western-Blot-NBP1-48319-img0004.jpg "Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319]")
Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319]
Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.![Immunocytochemistry/ Immunofluorescence: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319]](https://resources.bio-techne.com/images/products/GABA-A-R-beta-1-Antibody-S96-55-Immunocytochemistry-Immunofluorescence-NBP1-48319-img0003.jpg "Immunocytochemistry/ Immunofluorescence: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319]")
Immunocytochemistry/ Immunofluorescence: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319]
Immunocytochemistry/Immunofluorescence: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive.Applications for GABA-A R beta 1 Antibody (S96-55)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.1-1.0 ug/ml
Immunohistochemistry
1:1000
Western Blot
1-10 ug/ml
Application Notes
2 ug/ml of GABA-A Receptor Beta1 Antibody was sufficient for detection of Beta1 GABA receptor in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GABA-A R beta 1
Long Name
gamma-Aminobutyric Acid Type A Receptor, beta-1 Polypeptide
Alternate Names
GABAAR beta 1, GABAARb1, GABRB1
Gene Symbol
GABRB1
UniProt
Additional GABA-A R beta 1 Products
Product Documents for GABA-A R beta 1 Antibody (S96-55)
Product Specific Notices for GABA-A R beta 1 Antibody (S96-55)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...